Quantitative assessment of atherosclerotic lesion of rabbit aorta by compressed slice analysis of magnetic resonance images ex vivo

Byung HS. Cho, Denis Kholine, Byung H. Chung, Boris Odintsov


The objective of the present study was to evaluate a new compressed slice analysis technique of aortas ex vivo. Atherosclerosis was developed in rabbits by feeding an atherogenic diet containing 2% cholesterol and 6% peanut oil for 8 weeks. At termination, aortas were excised and imaged ex vivo by Magnetic resonance images (MRI). MRI data were acquired on a super high magnetic field vertical bore imaging scanner equipped with a Unity/Inova console, operating at 14.1 T and dedicated to small animal studies. T2-weighted coronal 2-D sections were acquired first to determine a ROI (region of interest) of the thoracic aorta using a Spin-Echo multi slice pulse sequence. Then, T2-weighted images were acquired in transversal sections of ROI slice by slice (2 mm/slice) in the total length of 4 cm. The MRI images of 20 slices were then compressed into a single image, and the resultant compressed single image was used as a quantitative characteristic of atherosclerotic lesions. By utilizing the compressed slices (CS) analysis, the imaging intensity of atherosclerotic lesions was calculated. A significant correlation was found between the quantitative CS-MRI and the aortic cholesterol content (Pearson correlation coefficient r = 0.95). The present findings demonstrate that the compressed slice analysis technique of MRI images could be applied for a quantitative assessment of the progression and/or regression of atherosclerosis.

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DOI: https://doi.org/10.5430/jbgc.v6n1p1

Journal of Biomedical Graphics and Computing    ISSN 1925-4008 (Print)   ISSN 1925-4016 (Online)

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