ARMS-PCR based diagnosis of APCB and KRAS genes in colorectal cancer patients in Iraqi Sulaimania province

Abstract

Amplification Refractory Mutation System (ARMS) assay is achieved for 30 blood samples of colorectal cancer (CRC) patients including 17 male and 13 female in order to determine the mutations in APCB gene and a proto-oncogene KRAS. ARMS-PCR analysis shows that all 30 samples are negative for I1307K variant (APCB) and seven common mutations in codons 12 and 13 (APCB). In addition two CRC patient samples are randomly chosen and purified for sequencing at Genome Center/KOYA University, then the sequence of the samples were BLAST on NCBI Website. The sequence alignment showed that both samples were wild for I1307K (APCB) and (G12S, G12C, G12A, G12D, G12V, G13D) (KRAS). We believe that these variants might absent in CRC patients of Iraqi Sulaimania province.